Ruodan Xu
China Academy of Chinese Medicine Science, China
Title: Fibrogenic of hiPS-MSCs in 3D connective tissue growth factor-delivering scaffold
Biography
Biography: Ruodan Xu
Abstract
Scarcity and damaged fibroblasts is one cause of impaired healing. We
developed a novel 3D biomimetic scaffold for stem cells augmentation
and differentiation to fibroblasts. The 3D scaffold PFPC-C in this
study was prepared using electrospun PCL/Col1 fibers which
embedded in PEG-fibrinogen (PF) hydrogel and infiltrated with
connective tissue growth factor (CTGF). In vitro, PFPC-C scaffold
showed good biocompatibility confirmed by the human induced
pluripotent stem cells derived mesenchymal stem cells (hiPS-MSCs)
attachment, infiltration, and proliferation throughout the construct.
CTGF loaded in this scaffold were sustained released and hiPS-MSCs
coultured on the scaffolds showed stronger fibroblastic commitment.
The performance of the scaffold was further evaluated in vivo. PFPC-C
scaffolds seeded with or without hiPS-MSCs were implanted in the rat
muscle injury model. 4 weeks, 12 weeks and 6 months later the mice
were sacrificed respectively, the grafted implants were analyzed with
histologic and mechanical examinations. Our results showed this 3D
scaffold provided a suitable environment and well fibroblasts
differentiation from hiPS-MSCs. This 3D scaffold could be potentially
used in cell-based therapies for connective tissue related disorders.